Peptide reconstitution and bacteriostatic water

This article describes a laboratory procedure for researchers. All information is intended solely for in vitro studies and does not constitute medical advice.

Sterile water for injection containing 0.9% benzyl alcohol. Benzyl alcohol inhibits bacterial growth, allowing the solution to be stored for up to 4 weeks at +4 °C.

Perform under sterile conditions.

1. Preparation: allow the vial with the powder and the BAC Water to warm to room temperature.
2. Disinfection: wipe the stoppers of both vials with an alcohol swab.
3. Drawing the solvent: draw the required volume of BAC Water into a sterile syringe.
4. Injection: inject the BAC Water along the wall of the vial — not directly onto the powder.
5. Mixing: gently roll the vial between your palms until dissolved. Do not shake.
6. Labeling: write down the date and concentration. Store at +4 °C or −20 °C.

Concentration (mg/mL) = mass of powder (mg) / volume of solvent (mL)

Example: 5 mg + 2 mL BAC Water = 2.5 mg/mL.