GHK-Cu: dosing evidence
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Calculate the GHK-Cu dose
The calculator is already set up for GHK-Cu — adjust the dose or diluent volume for your vial.
Human dosing
GHK-Cu dosing in the schedule below climbs gradually, from 1 mg daily in the first two weeks to 2 mg later in the cycle, rather than starting at the upper bound: this peptide's effect depends on concentration in a non-linear way.
| Period / condition | Dose | Escalation |
|---|---|---|
| Start (≈first 2 weeks) | 1 mg subcutaneously daily, ~5 days/week | Starting dose |
| Later within the cycle | 2 mg subcutaneously daily | Increase if needed |
| Body-weight reference | 15–30 mcg/kg daily | Maximum in this schedule |
Practical dosing and effect details
A review by Pickart and Margolina (International Journal of Molecular Sciences, 2018, volume 19) cites cell-culture data: GHK-Cu at 0.01, 1, and 100 nM, added to human dermal fibroblasts, raised collagen and elastin production at all three levels, while MMP1 and MMP2 gene expression rose specifically at the lowest, 0.01 nM, concentration.
These are cell-culture, nanomolar concentrations that cannot be equated directly with the milligram injected doses on this page: the units and context differ. But the underlying principle is the same and explains why the schedule here does not sit at the maximum from the start: the gradual escalation from 1 mg to 2 mg daily, with a 15-30 mcg/kg reference, reflects that GHK-Cu's effect does not necessarily scale in direct proportion to the dose.
This is reference information for planning a research protocol, not a medical recommendation and not instructions for self-administration.
How to reconstitute
An example reconstitution for convenient dosing. The exact ratios depend on your vial. For research reference only — not medical guidance.
- 1Add the diluentUse bacteriostatic water. Example: 2 ml into a 50 mg vial.GHK-Cu50 mg+ 2 mlDiluent
- 2Get the concentrationExample: 50 mg in 2 ml =GHK-Cu25 mg/ml(25,000 mcg/ml)
- 3Work out the volume per doseFor example, for a 1 mg dose:1 mg = 0.04 ml= 4 u. (U-100)
This is only an example calculation. You can use a different diluent volume — plug your own numbers into the calculator. Open calculator →
Research protocols
GHK-Cu: reference regimens
As an injectable reagent, GHK-Cu is used subcutaneously (SC), typically into soft abdominal tissue.
More scientific background in the GHK-Cu monograph
Notes and safety
- • GHK-Cu is a research peptide. Human safety and efficacy data are limited.
- • The regimens shown are based on publications and research protocols, not clinical recommendations.
- • Individual reactions may vary. Published protocols typically start dosing at the lower end of the reported range.
- • Not recommended for use with chronic illness, pregnancy, or breastfeeding.
- • This information is for educational purposes and is not medical advice.
