NAD+

NAD+ (nicotinamide adenine dinucleotide) is the coenzyme behind virtually every serious study of cellular energy metabolism and aging. It shuttles electrons through hundreds of redox reactions and is the obligate substrate for the sirtuin enzymes (SIRT1–7) and PARP, the very proteins that link NAD+ status to mitochondrial function and cellular aging. Tissue NAD+ levels decline with age, which is why this molecule remains one of the most frequently studied targets in longevity research.

Sample format and lab handling

Supplied as a lyophilized powder in a glass vial carrying an RUO (research use only) label. Every batch is checked by HPLC, which makes the material suitable for reproducible laboratory protocols. A certificate of analysis (COA) is tied to the batch number.

Keep the vial tightly closed, in a dry place, protected from light; low temperatures are recommended for long-term storage. Reconstituted NAD+ is unstable at room temperature, so standard lab practice is to prepare small volumes immediately before use and keep the solution chilled until the work is finished. The solvent, concentration, and shelf life of the reconstituted solution are set by your lab's own SOP.

Research context: sirtuins, PARP, and the precursor pathway

Beyond direct administration of the finished molecule, cellular NAD+ is also replenished through precursors: nicotinamide riboside (NR) and nicotinamide mononucleotide (NMN) are converted into NAD+ through an enzymatic pathway and form their own branch of longevity research. The Longeva catalog carries that pathway as a separate sample: nicotinamide riboside (NR). The conversion mechanism from precursor to NAD+ is covered in the wiki overview of the NR/NMN pathway.

Verifying a batch against its COA

1. Find the LOT number on the vial and outer packaging.
2. Open the PDF for this substance in the COA archive.
3. Check the substance name, methods (HPLC, identity), and result units.
4. Record the receipt date and the PDF link in your batch log.

COAs are issued by independent third-party labs; a report confirms the analytics at the time of testing, not a medical status for the material.

Frequently asked questions

How does NAD+ differ from NR and NMN? NAD+ is already the finished form of the coenzyme, while NR and NMN are precursors that the cell converts into NAD+ through an enzymatic pathway. A comparison of these forms across research models is covered in the linked wiki monograph.

What does HPLC batch testing show? High-performance liquid chromatography confirms substance identity and measures batch purity against a reference standard; the result is recorded in that batch's COA.

Where is the full scientific writeup and primary-source references? Molecular structure, the redox reaction mechanism, and the primary-source reference list are set out in the linked NAD+ wiki monograph.

Further reading

Reference material. Not medical advice. This sample is intended for laboratory research use only (RUO).